A program that matches
primers to a DNA sequence

Requirements
Navigate your primer collection by sorting primers by sequence
Download PrimerStacka 1.4 MB program for both Mac's and PC's
See if you need JavaVersion 8 or later of JRE required
< a free and easy download >

Features

Flexible Matching

  • Align primers to a sequence using mismatches, insertions, and deletions.
  • Easily see where and how well each primer binds to the DNA!

Customizable GenBank

  • Mark up a sequence with genes, binding sites, transposon insertions, and any other features you need to see.
  • Use different colors for different non-gene features!

Primer Design

  • Automatically generate many primers that match your sequence.
  • Design primers based on your desired Tm and length.
  • Input your primer and salt concentrations or use PCR conditions.

Screenshots

Click on tabs and buttons with red arrows

Download PrimerStacka 1.4 MB program for both Mac's and PC's
See if you need JavaVersion 8 or later of JRE required
< a free and easy download >